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用于慢性病大型哺乳动物心脏模型对比增强显影显微计算机断层扫描的组织制备技术

JoVE Journal
Medicine

This content is Free Access.

JoVE Journal Medicine

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Please note that all translations are automatically generated. Click here for the English version.

用于慢性病大型哺乳动物心脏模型对比增强显影显微计算机断层扫描的组织制备技术

DOI:

10.3791/62909-v

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12:15 min

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February 08, 2022

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Néstor Pallares-Lupon², Jason D. Bayer², Bastien Guillot², Guido Caluori², Girish S. Ramlugun², Kanchan Kulkarni², Virginie Loyer², Stephane Bloquet², Dounia El Hamrani², Jérôme Naulin², Marion Constantin², Pierre Dos Santos^2,3, Olivier Bernus², Pierre Jaïs^2,3, Philippe Pasdois², Richard D. Walton²

¹Centre de recherche Cardio-Thoracique de Bordeaux,Univ. Bordeaux, ²IHU Liryc, Electrophysiology and Heart Modeling Institute,Fondation Bordeaux Univ., ³Electrophysiology and Ablation Unit,Bordeaux University Hospital (CHU)

Chapters

00:04Introduction
00:54Solution Preparation
02:05Tissue Preparation
05:21Tissue Dehydration and Drying
06:57MicroCT
09:20Results: Contrast-Enhanced Air-Dried Tissue Preparations Enabled Microscale Imaging of Intact Large Mammalian Cardiac Models with Chronic Disease
11:36Conclusion

Summary

Automatic Translation

在这里，我们提出了一种方案，以获得健康和病理性的大型哺乳动物全心的高分辨率微计算机断层扫描图像，并具有胶原选择性对比度增强。

Tags

Keywords: Micro Computed Tomography Cardiac Models Chronic Disease Contrast Enhancement Large Mammalian Microstructure Fibrosis Arrhythmia Cardioplegic Solution PBS/EDTA PMA Contrast Agent Coronary Osteo PTFE Tubing

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