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Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
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JoVE Journal Neuroscience
Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

DOI:
10.3791/62932-v

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07:14 min

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February 25, 2022

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, ,
1Department of Chemical Biology,Tokyo Medical University, 2Division of Molecular and Developmental Biology,National Institute of Genetics, 3Department of Genetics,Graduate University for Advanced Studies (SOKENDAI)

Chapters

  • 00:04Introduction
  • 01:36Preparation of Light Emitting Diode (LED) for Blue Light Illumination
  • 02:13Imaging of Zebrafish Larvae Expressing Optogenetic TAR DNA-binding Protein 43 (TDP-43)
  • 03:58Light Stimulation of Optogenetic TDP-43h-Expressing Fish by Field Illumination of a Blue LED Light
  • 04:35Results: Characterization of TDP-43 Phase Transition in Spinal Motor Neurons of Zebrafish Larvae
  • 06:34Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model.

Tags

Keywords: OptogeneticTDP-43Spinal Motor NeuronsZebrafish LarvaeALSPhase TransitionProtein AggregationIntrinsically Disordered RegionsBAC TransgenesisLEDOptical Power MeterAgaroseAnesthesia

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