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Biochemistry

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使用噬菌体显示开发用于E3连接酶的泛素变体调节剂
 
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使用噬菌体显示开发用于E3连接酶的泛素变体调节剂

Article DOI: 10.3791/62950-v 06:30 min August 27th, 2021
August 27th, 2021

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Summary

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泛素化是翻译后修饰的关键蛋白质,其失调与许多人类疾病有关。该协议详细介绍了如何利用噬菌体显示来分离新的泛素变体,这些变体可以结合和调节控制泛素特异性,效率和模式的E3连接酶的活性。

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生物化学,第174期,
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