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Biochemistry
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使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量
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Biochemistry
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JoVE Journal
Biochemistry
Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
Please note that all translations are automatically generated.
Click here for the English version.
使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量
DOI:
10.3791/62608-v
•
11:38 min
•
September 14, 2021
•
Paul-Lennard Mendez
2
,
Leon Obendorf
,
Petra Knaus
1
Institute for Chemistry and Biochemistry
,
Freie Universität Berlin
,
2
International Max-Planck Research School for Biology and Computation
Chapters
00:05
Introduction
00:40
Cell Culture and Fluid Shear Stress Exposure
02:55
Fixation
04:58
Blocking and Incubation with Primary Antibody
06:28
Incubation with PLA Probe and Ligation
07:34
Amplification and Mounting
09:14
Results: Effect of Shear Stress on the SMAD Protein Interactions
11:02
Conclusion
Summary
Automatic Translation
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在这里,我们建立了一个方案,使用暴露于病理和生理流体剪切应激条件下的内皮细胞中的接近连接测定(PLA)同时可视化和分析多个SMAD复合物。
Tags
TGF-β/BMP/SMAD Signaling
Proximity Ligation Assay
Fluid Shear Stress
Microfluidic Flow Channels
HUVEC Cells
Transcription Factor Complexes
Vascular Biology
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