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使用接近-连接-测定法对不同流体剪切应力条件下TGFβ/BMP/SMAD信号传导的可视化和定量
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
DOI:

11:38 min

September 14, 2021

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Chapters

  • 00:05Introduction
  • 00:40Cell Culture and Fluid Shear Stress Exposure
  • 02:55Fixation
  • 04:58Blocking and Incubation with Primary Antibody
  • 06:28Incubation with PLA Probe and Ligation
  • 07:34Amplification and Mounting
  • 09:14Results: Effect of Shear Stress on the SMAD Protein Interactions
  • 11:02Conclusion

Summary

Automatic Translation

在这里,我们建立了一个方案,使用暴露于病理和生理流体剪切应激条件下的内皮细胞中的接近连接测定(PLA)同时可视化和分析多个SMAD复合物。

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