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Journal / Biochemistry / Prøvepreparering og relativ kvantrasjon ved bruk…
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Biochemistry

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JoVE Journal Biochemistry
Prøvepreparering og relativ kvantrasjon ved bruk av reduktiv metylering av aminer for peptidomikkstudier
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Prøvepreparering og relativ kvantrasjon ved bruk av reduktiv metylering av aminer for peptidomikkstudier

Article DOI: 10.3791/62971-v • 08:00 min • November 4th, 2021
November 4th, 2021 •
1,2, 1, 1, 1,2
1Department of Biological and Environmental Sciences, Bioscience Institute, Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute, Sao Paulo State University (UNESP)

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Denne artikkelen beskriver en prøveprepareringsmetode basert på varmeinaktivering for å bevare endogene peptider som unngår nedbrytning etter mortem, etterfulgt av relativ kvantisering ved hjelp av isotopisk merking pluss LC-MS.

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