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JoVE Journal Biochemistry
Probenvorbereitung und relative Quantifizierung mittels reduktiver Methylierung von Aminen für Peptidomik-Studien
Single Particle Cryo-Electron Microscopy: From Sample to Structure
Journal (Biochemistry)
Single Particle Cryo-Electron Microscopy: From Sample to Structure
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
 
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Probenvorbereitung und relative Quantifizierung mittels reduktiver Methylierung von Aminen für Peptidomik-Studien

Article DOI: 10.3791/62971-v • 08:00 min • November 4th, 2021
November 4th, 2021 •
1,2, 1, 1, 1,2
1Department of Biological and Environmental Sciences, Bioscience Institute, Sao Paulo State University (UNESP), 2Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute, Sao Paulo State University (UNESP)

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Dieser Artikel beschreibt eine Probenvorbereitungsmethode, die auf Wärmeinaktivierung basiert, um endogene Peptide zu erhalten und den Abbau post mortem zu vermeiden, gefolgt von einer relativen Quantifizierung mittels Isotopenmarkierung plus LC-MS.

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Biochemie Heft 177
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