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Biochemistry
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JoVE Journal Biochemistry
Prøvepreparering ved hjelp av en Lipid Monolayer-metode for elektronkrystallografiske studier
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Prøvepreparering ved hjelp av en Lipid Monolayer-metode for elektronkrystallografiske studier

Article DOI: 10.3791/63015-v • 04:22 min • November 20th, 2021
November 20th, 2021 •
1,2, 3, 1, 4, 1,2
1School of Molecular Sciences, Arizona State University, 2Center for Applied Structural Discovery, Biodesign Institute, Arizona State University, 3Eyring Materials Center, Arizona State University, 4Department of Microbiology and Immunology, The Pennsylvania State University

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Lipidmonolayers har blitt brukt som grunnlag for å danne todimensjonale (2D) proteinkrystaller for strukturelle studier i flere tiår. De er stabile ved luftvannsgrensesnittet og kan fungere som et tynt støttemateriale for elektronavbildning. Her presenterer vi de påviste trinnene for å forberede lipidmonolayers for biologiske studier.

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