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Biochemistry

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JoVE Journal Biochemistry
Micropatterning Transmissie ElektronenMicroscopie Grids naar Direct Cell Positioning binnen Whole-Cell Cryo-Electron Tomography Workflows
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Micropatterning Transmissie ElektronenMicroscopie Grids naar Direct Cell Positioning binnen Whole-Cell Cryo-Electron Tomography Workflows

Article DOI: 10.3791/62992-v • 09:53 min • September 13th, 2021
September 13th, 2021 •
*1,2,3, *1,4, 1,2,3, 1,2,3,5
1Department of Biochemistry, University of Wisconsin, Madison, 2Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, 3Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, 4Department of Chemistry, University of Wisconsin, Madison, 5Morgridge Institute for Research
* These authors contributed equally

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Het doel van dit protocol is om celadhesie en -groei te richten op gerichte gebieden van rasters voor cryo-elektronenmicroscopie. Dit wordt bereikt door een aangroeiwerende laag aan te brengen die wordt opgeblazen in door de gebruiker gespecificeerde patronen, gevolgd door afzetting van extracellulaire matrixeiwitten in de patroongebieden voorafgaand aan het zaaien van cellen.

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Biochemie celkweek maskerloze fotopatterning micropatterning cryo-elektronenmicroscopie cryo-EM cryo-elektronentomografie cryo-ET correlatieve licht- en elektronenmicroscopie CLEM fluorescentielichtmicroscopie fLM
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