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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
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Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Article DOI: 10.3791/63045-v • 10:17 min • September 24th, 2021
September 24th, 2021 •
1, 2, 2,3,4, 1,4,5
1Department of Pathology, University of Michigan, Ann Arbor, 2Department of Internal Medicine, University of Michigan, Ann Arbor, 3Department of Cellular and Developmental Biology, University of Michigan, Ann Arbor, 4Rogel Cancer Center, University of Michigan, Ann Arbor, 5Institute of Gerontology, University of Michigan, Ann Arbor

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Summary
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The method presented here summarizes optimized protocols for assessing cellular bioenergetics in non-adherent mouse hematopoietic stem and primitive progenitor cells (HSPCs) using the extracellular flux analyzer to measure the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) of HSPCs in real time.

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Cellular Bioenergetics Mouse Hematopoietic Stem Cells Glycolysis Oxidative Phosphorylation Extracellular Flux Analyzer Mitochondrial Functions High Throughput Quantification Metabolic Pathways HSC Metabolism Suspension Cells Assay Sensor Cartridge Utility Plate Assembly Calibrant
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