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Coltura e imaging di organoidi epiteliali nasali umani
JoVE Journal
Medicine
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JoVE Journal Medicine
Culture and Imaging of Human Nasal Epithelial Organoids
DOI:

13:20 min

December 17, 2021

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Chapters

  • 00:04Introduction
  • 01:40Cell Culture and Expansion of Nasal Epithelial Cells
  • 04:23Growth and Differentiation of Organoids in Slides and Culture Inserts
  • 06:06Isolation and Fixation of Organoids
  • 07:36Preparation and Isolation of Organoids for Histological Sectioning
  • 08:57Baseline Lumen Measurements
  • 09:56Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods
  • 12:21Conclusion

Summary

Automatic Translation

Un protocollo dettagliato è presentato qui per descrivere un modello organoide in vitro da cellule epiteliali nasali umane. Il protocollo ha opzioni per le misurazioni che richiedono apparecchiature di laboratorio standard, con possibilità aggiuntive per attrezzature e software specializzati.

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