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JoVE Journal
Medicine

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Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
 

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

Article DOI: 10.3791/50796
May 18th, 2014

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Summary May 18th, 2014

A protocol to isolate, culture, and image islet cell clusters (ICCs) derived from human fetal pancreatic cells is described.  The method details the steps necessary to generate ICCs from tissue, culture as monolayers or in suspension as aggregates, and image for markers of proliferation and pancreatic cell fate decisions.

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