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Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters

DOI:
10.3791/50796-v

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08:09 min

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May 18, 2014

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, , ,
1Pediatric Diabetes Research Center, Department of Pediatrics,University of California, San Diego

Chapters

  • 00:05Title
  • 01:20Dissection of Human Fetal Pancreas
  • 03:48Immunofluorescence and Maturation of Islet Cell Clusters
  • 05:36Results: Isolated, Cultured, and Imaged Human Fetal Pancreatic Cell Clusters
  • 07:34Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol to isolate, culture, and image islet cell clusters (ICCs) derived from human fetal pancreatic cells is described.  The method details the steps necessary to generate ICCs from tissue, culture as monolayers or in suspension as aggregates, and image for markers of proliferation and pancreatic cell fate decisions.

Tags

Keywords: Human Fetal Pancreatic Cell ClustersInsulin Producing CellsEndocrine Precursor CellsHESCIn Vitro GenesisC-peptidePancreatic DevelopmentPancreatic EndodermPancreatic MarkersGlucagon

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