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Konfektavbildning av Quantum Dot-Konjugert SARS-CoV-2 spike trimers for å spore binding og endokytose i HEK293T-celler
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Konfektavbildning av Quantum Dot-Konjugert SARS-CoV-2 spike trimers for å spore binding og endokytose i HEK293T-celler

Article DOI: 10.3791/63202-v • 06:39 min • April 21st, 2022
April 21st, 2022 •
1, 2, 2,3, 2, 1
1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation

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I denne protokollen, kvanteprikker konjugert til rekombinant SARS-CoV-2 spike aktivere cellebaserte analyser for å overvåke spike binding til hACE2 ved plasmamembranen og påfølgende endokytose av de bundne proteiner i cytoplasma.

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