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JoVE Journal
Biochemistry

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体外 使用分析、下拉和伴侣测定表征组蛋白伴侣
 
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体外 使用分析、下拉和伴侣测定表征组蛋白伴侣

Article DOI: 10.3791/63218-v 08:16 min December 29th, 2021
December 29th, 2021

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Summary

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Click here for the English version.

该协议描述了一系列方法,包括分析体积排阻色谱以研究组蛋白伴侣寡聚化和稳定性,下拉测定以揭示组蛋白伴侣 - 组蛋白相互作用,AUC用于分析蛋白质复合物的化学计量,以及组蛋白伴侣测定以在 体外功能表征假定的组蛋白伴侣。

Tags

生物化学,第178期,组蛋白伴侣,核小体组装,染色质,核蓝蛋白,NAP,质粒超螺旋,分析超速离心
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