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Journal / Biochemistry / 基于无标签阻抗的 GPCR…
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Biochemistry

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JoVE Journal Biochemistry
基于无标签阻抗的 GPCR 检测中用于提高吞吐量的逐步加量协议
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基于无标签阻抗的 GPCR 检测中用于提高吞吐量的逐步加量协议

Article DOI: 10.3791/60686-v • 06:13 min • February 21st, 2020
February 21st, 2020 •
1, 1, 1, 1, 2, 2, 3, 3, 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

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该协议演示了使用无标签阻抗测量从单个微电极上生长的单个细胞层的激动剂诱导GPCR活化的全剂量-反应关系的实时记录。新的配量方案显著提高了吞吐量，而不会损失时间分辨率。

Tags

生物化学，问题156，G-蛋白耦合受体（GPCR），阻抗，无标签，基于细胞的测定，浓度-反应关系，激动剂，拮抗剂，组胺
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