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Journal / Medicine / Transkriptom Düzeyinde İnsan Primer Mezenterik…
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Transkriptom Düzeyinde İnsan Primer Mezenterik Arteriyel Endotel Hücrelerinin İzolasyonu ve Profillenmesi
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Transkriptom Düzeyinde İnsan Primer Mezenterik Arteriyel Endotel Hücrelerinin İzolasyonu ve Profillenmesi

Article DOI: 10.3791/63307-v • 09:45 min • March 14th, 2022
March 14th, 2022 •
*1, *1, *1, 1,3, 2, 2, 1,3
1Department of Diabetes Complications and Metabolism, City of Hope, 2Department of Bioengineering, University of California San Diego, 3Irell and Manella Graduate School of Biological Sciences, City of Hope
* These authors contributed equally

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Protokol, endotel hücrelerinin insan mezenterik arterinden izolasyonunu, kültürünü ve profillemesini tanımlar. Ek olarak, insan arterini mekansal transkriptomik için hazırlamak için bir yöntem sağlanmıştır. İzole hücreler üzerinde proteomik, transkriptomik ve fonksiyonel testler yapılabilir. Bu protokol, herhangi bir orta veya büyük boyutlu arter için yeniden kullanılabilir.

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