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Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice
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Chapters
- 00:04Introduction
- 00:52Positioning of the Mouse in the Stereotaxic Frame
- 02:04Surgery to Access the Cisterna Magna
- 03:34Opening of the Cisternal Membrane and Identification of Landmarks and Zero Point
- 04:11Injection of the Target
- 04:45Closure of the Surgical Field
- 05:10Results: Stereotaxic Surgical Approach in Mice
- 06:14Conclusion
Stereotaxic surgery to target brain sites in mice commonly involves access through the skull bones and is guided by skull landmarks. Here we outline an alternative stereotaxic approach to target the caudal brainstem and upper cervical spinal cord via the cisterna magna that relies on direct visualization of brainstem landmarks.
