Journal
/
Biochemistry
/
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

DOI:
10.3791/63377-v

•

07:20 min

•

February 18, 2022

•

, ,
1Molecular Biology,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Chapters

  • 00:04Introduction
  • 01:05Microtubule Visualization via TIRF Microscopy
  • 01:58Microscope Settings
  • 03:17Generation of Surface-Immobilized Microtubule Bundles
  • 04:57Image Microtubule Dynamics
  • 05:53Results: TIRF Microscopy of Crosslinked and Single Microtubules
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a TIRF microscopy-based in vitro reconstitution assay is presented to simultaneously quantify and compare the dynamics of two microtubule populations. A method is described to simultaneously view the collective activity of multiple microtubule-associated proteins on crosslinked microtubule bundles and single microtubules.

Tags

MicrotubulesTIRF MicroscopyMulti-component ReconstitutionMicrotubule DynamicsMicrotubule SubpopulationsSingle MicrotubulesMicrotubule BundlesFluorescent LabelingExperimental ParametersImaging Setup

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level

Imaging of Extracellular Vesicles by Atomic Force Microscopy

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Visualizing Diffusional Dynamics of Gold Nanorods on Cell Membrane using Single Nanoparticle Darkfield Microscopy

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Quantitative Microtubule Fractionation Technique to Separate Stable Microtubules, Labile Microtubules, and Free Tubulin in Mouse Tissues

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Read Article