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JoVE Journal Biochemistry
Samtidig visualisering av dynamikken i krysskoblede og enkle mikrotubuler in vitro av TIRF mikroskopi
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Samtidig visualisering av dynamikken i krysskoblede og enkle mikrotubuler in vitro av TIRF mikroskopi

Article DOI: 10.3791/63377-v • 07:20 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1, 1,2
1Molecular Biology, Massachusetts General Hospital, 2Department of Genetics, Harvard Medical School
* These authors contributed equally

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Her presenteres en TIRF-mikroskopibasert in vitro rekonstitueringsanalyse for samtidig å kvantifisere og sammenligne dynamikken i to mikrotubulpopulasjoner. En metode er beskrevet for samtidig å se den kollektive aktiviteten til flere mikrotubule-assosierte proteiner på krysskoblede mikrotubulbunter og enkle mikrotubuler.

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