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En reporterassay for at analysere intronisk mikroRNA-modning i pattedyrceller
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En reporterassay for at analysere intronisk mikroRNA-modning i pattedyrceller

Article DOI: 10.3791/63498-v • 06:48 min • June 16th, 2022
June 16th, 2022 •
1, 1
1Departamento de Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade de São Paulo

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Vi udviklede et intronisk mikroRNA biogenese reporterassay til brug i celler in vitro med fire plasmider: en med intronisk miRNA, en med målet, en til at overudtrykke et regulatorisk protein og en til Renilla luciferase. MiRNA'et blev behandlet og kunne kontrollere luciferaseekspression ved at binde til målsekvensen.

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Kræftforskning nummer 184
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