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一种报告基因检测法,用于分析哺乳动物细胞中内旋微RNA成熟
 
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一种报告基因检测法,用于分析哺乳动物细胞中内旋微RNA成熟

Article DOI: 10.3791/63498-v 06:48 min June 16th, 2022
June 16th, 2022

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我们开发了一种内旋microRNA生物发生报告基因检测方法,用于具有四种质粒 的体外 细胞:一种具有内旋性miRNA,一种具有靶标,一种用于过表达调节蛋白,另一种用于Renilla荧光素酶。miRNA经过处理,可以通过与靶序列结合来控制荧光素酶的表达。

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癌症研究,第184期,
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