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Biochemistry

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JoVE Journal Biochemistry
使用200 KV透射电子显微镜常规收集高分辨率冷冻电镜数据集
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
Journal (Biochemistry)
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
Journal (Biochemistry)
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
 
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使用200 KV透射电子显微镜常规收集高分辨率冷冻电镜数据集

Article DOI: 10.3791/63519-v • 09:49 min • March 16th, 2022
March 16th, 2022 •
*1, *2, 1, 1, 1, 1, 1, 1, 1, 3
1Materials and Structural Analysis Division, Thermo Fisher Scientific, 2Max Planck Institute of Biochemistry, Molecular Structural Biology, 3Materials and Structural Analysis Division, Thermo Fisher Scientific
* These authors contributed equally

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大分子的高分辨率冷冻电镜图谱也可以通过使用200 kV透射显微镜来实现。该协议显示了设置精确光学对准、数据采集方案和成像区域选择的最佳实践，这些对于使用200 kV TEM成功收集高分辨率数据集至关重要。

Tags

生物化学，第181期，冷冻电镜，高分辨率，结构，对齐，数据收集，数据管理，冰川，EPU，能量过滤器
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