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Journal / Biology / En fluorescensbaserad analys av membranpotential…
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JoVE Journal Biology
En fluorescensbaserad analys av membranpotential för funktionell studie med hög kapacitet av två endogena jonkanaler i två epitelcellinjer
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En fluorescensbaserad analys av membranpotential för funktionell studie med hög kapacitet av två endogena jonkanaler i två epitelcellinjer

Article DOI: 10.3791/63528-v • 06:59 min • June 22nd, 2022
June 22nd, 2022 •
1,3, 3, 2,4,5, 1,3,5
1Molecular Medicine, Hospital for Sick Children, 2Cell Biology, Hospital for Sick Children, 3Department of Physiology, University of Toronto, 4Department of Paediatrics, University of Toronto, 5Department of Biochemistry, University of Toronto

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Detta protokoll beskriver en metod för studier av elektrogena membranproteiner genom att mäta förändringar i membranpotential. Denna analys ger en plattform för funktionell avläsning av flera jonkanaler endogent uttryckta i epitelcellinjer.

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Denna månad i JoVE nummer 184
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