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Bioengineering
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使用拉曼染料进行高度多重组织成像
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Highly-Multiplexed Tissue Imaging with Raman Dyes
Please note that all translations are automatically generated.
Click here for the English version.
使用拉曼染料进行高度多重组织成像
DOI:
10.3791/63547-v
•
07:18 min
•
April 21, 2022
•
Lixue Shi
,
Mian Wei
,
Wei Min
2
1
Department of Chemistry
,
Columbia University
,
2
Kavli Institute for Brain Science
,
Columbia University
Chapters
00:04
Introduction
00:59
Preparation of Raman-Dye-Conjugated Antibodies
02:57
Tissue Immuno-eprSRS Staining
05:02
Image Acquisition and Analysis
05:35
Results: Electronic Pre-Resonance Stimulated Raman Scattering Imaging of Raman Dyes
06:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
彩虹状拉曼染料的电子预共振受激拉曼散射(epr-SRS)成像是高度多重表位蛋白质成像的新平台。在这里,我们提供了一个实用指南,包括抗体制备,组织样品染色,SRS显微镜组装和epr-SRS组织成像。
Tags
Highly-multiplexed
Vibrational Imaging
Protein Markers
Subcellular Resolution
Cycle-free Multiplexity
Cell Characterization
Tumor Microenvironments
Brain Circuit
Sodium Bicarbonate
PBS Buffer
N-hydroxysuccinimide Ester
MARS Probe
Antibody Conjugation
Size Exclusion Column
Purification
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