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JoVE Journal
Bioengineering

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Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Article DOI: 10.3791/2592
May 18th, 2011 •
*1, *1, 1, 1,2
1Center for Biophotonics Science and Technology, University of California, Davis, 2Department of Pathology and Laboratory Medicine, University of California, Davis
* These authors contributed equally

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May 18th, 2011

We discuss the construction and operation of a complex nonlinear optical system that uses ultrafast all-optical switching to isolate Raman from fluorescence signals. Using this system we are able to successfully separate Raman and fluorescence signals utilizing pulse energies and average powers that remain biologically safe.

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