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A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator
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Bioengineering
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JoVE Journal Bioengineering
A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator

A Reliable and Reproducible Critical-Sized Segmental Femoral Defect Model in Rats Stabilized with a Custom External Fixator

DOI:
10.3791/59206-v

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08:20 min

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March 24, 2019

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, , , , , , ,
1Department of Orthopedics and Rehabilitation,University of Wisconsin-Madison, 2Department of Biomedical Engineering,University of Wisconsin-Madison, 3Summit Orthopedics

Chapters

  • 00:04Title
  • 00:58Bone Morphogenetic Protein-2 (rhBMP-2)-Soaked Sponge Scaffold and Surgical Preparation
  • 01:54Segmental Femoral Defect Induction
  • 05:50Results: Representative Segmental Femoral Defect Bone Recovery Analyses
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

In vivo mammalian models of critical-sized bone defects are essential for researchers studying healing mechanisms and orthopedic therapies. Here, we introduce a protocol for the creation of reproducible, segmental, femoral defects in rats stabilized using external fixation.

Tags

Keywords: Segmental Femoral DefectRat ModelExternal FixatorBone RegenerationBone Morphogenetic ProteinSurgical ProtocolCritical-sized DefectDiaphyseal DefectLongitudinal Healing Studies

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