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High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

DOI:
10.3791/63649-v

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09:31 min

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April 28, 2022

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, , , ,
1Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering,Hong Kong University of Science and Technology

Chapters

  • 00:04Introduction
  • 00:55Open-Top Ultraviolet Photoacoustic Microscopy (UV-PAM) System
  • 05:10Sample Preparation
  • 06:17Experimental Procedures
  • 07:57Results: Ultraviolet Photoacoustic Microscopy (UV-PAM) Imaging with Deep Learning-Based Virtual Staining
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental procedures.

Tags

Keywords: Ultraviolet Photoacoustic MicroscopyHistological ImagingVirtual StainingDeep LearningIntraoperative Surgical MonitoringTumor ResectionPositive Surgical MarginQ-switch Diode-pumped Solid-state LaserGalvanometer MirrorUltrasound TransducerData AcquisitionSample HolderAlignment

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