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Journal / Bioengineering / High-Speed Ultraviolet Photoacoustic Microscopy…
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Bioengineering

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JoVE Journal Bioengineering
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High-Speed Ultraviolet Photoacoustic Microscopy for Histological Imaging with Virtual-Staining assisted by Deep Learning

Article DOI: 10.3791/63649-v • 09:31 min • April 28th, 2022
April 28th, 2022 •
1, 1, 1, 1, 1
1Translational and Advanced Bioimaging Laboratory, Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology

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Summary
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A high-speed and open-top ultraviolet photoacoustic microscope that can provide histological images intraoperatively for surgical margin analysis is demonstrated, including the system configuration, optical alignment, sample preparation, and experimental procedures.

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High-speed Ultraviolet Photoacoustic Microscopy Histological Imaging Virtual-staining Deep Learning Intraoperative Surgical Monitor Analysis Tumor Resection Surgeries Diagnostic Tool Positive Surgical Margin Labeled Histological Images Imaging Area Xiufeng Li Q-switch Diode-pumped Solid-state Laser Convex Lenses Pinhole Spatial Filtering 1D Galvanometer Mirror Objective Lens Lab Made Water Tank
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