Journal
/
/
Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping
DOI:

12:19 min

December 08, 2015

, , , ,

Chapters

  • 00:05Title
  • 02:03Initializing the Microscope
  • 03:05Capturing Images
  • 04:13Capturing Datacubes
  • 06:07Creation of Reference Spectral Libraries
  • 07:59Image Analysis: Spectral Angle Mapping
  • 10:04Results: Hyperspectral Microscope Positively Identifies Nanoparticles
  • 11:25Conclusion

Summary

Automatic Translation

Enhanced darkfield microscopy and hyperspectral imaging with spectral mapping enable screening, localization, and identification of nanoscale materials in histological samples with improved speed and accuracy over traditional methods. The goal of this paper is to provide methods for darkfield imaging and hyperspectral mapping of metal oxide nanoparticles in histological samples.

Related Videos

Read Article