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Journal / Neuroscience / In situ Hybridisering kombinert med…
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
In situ Hybridisering kombinert med immunhistokjemi i kryoseksjonerte sebrafiskembryoer
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In situ Hybridisering kombinert med immunhistokjemi i kryoseksjonerte sebrafiskembryoer

Article DOI: 10.3791/63715-v • 07:36 min • March 3rd, 2022
March 3rd, 2022 •
*1,2, *3, 2, 4, 2, 1, 2,3, 5, 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University
* These authors contributed equally

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Denne protokollen beskriver hvordan man kan ta bilder ved å kombinere in situ hybridisering og immunhistokjemi av sebrafiskens embryonale seksjoner. In situ hybridisering ble utført før kryoseksjonering, etterfulgt av antistofffarging. Det er nyttig å påvise uttrykksmønstre av to gener hos sebrafisk hvis det er mangel på antistoffer.

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Denne måneden i JoVE utgave 181 in situ hybridisering immunhistokjemi sebrafisk mRNA antistoff protein
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