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Immunology and Infection

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JoVE Journal Immunology and Infection
Standardiserede in vitro-assays til visualisering og kvantificering af interaktioner mellem humane neutrofiler og Staphylococcus aureus biofilm
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Standardiserede in vitro-assays til visualisering og kvantificering af interaktioner mellem humane neutrofiler og Staphylococcus aureus biofilm

Article DOI: 10.3791/63773-v • 07:28 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1,2
1Department of Microbiology, The Ohio State University, 2Department of Microbial Infection and Immunity, The Ohio State University

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Den nuværende protokol beskriver undersøgelsen af neutrofil-biofilminteraktioner. Staphylococcus aureus biofilm etableres in vitro og inkuberes med perifere blodafledte humane neutrofiler. Det oxidative burstrespons fra neutrofiler kvantificeres, og neutrofillokaliseringen i biofilmen bestemmes ved mikroskopi.

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Immunologi og infektion udgave 184 biofilm neutrofiler ROS-produktion mikroskopi værtspatogeninteraktioner
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