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Immunology and Infection

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JoVE Journal Immunology and Infection
Standardiserte in vitro-analyser for å visualisere og kvantifisere interaksjoner mellom humane nøytrofiler og Staphylococcus aureus biofilmer
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Journal (Immunology and Infection)
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
Journal (Immunology and Infection)
A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
 
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Standardiserte in vitro-analyser for å visualisere og kvantifisere interaksjoner mellom humane nøytrofiler og Staphylococcus aureus biofilmer

Article DOI: 10.3791/63773-v • 07:28 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1,2
1Department of Microbiology, The Ohio State University, 2Department of Microbial Infection and Immunity, The Ohio State University

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Denne protokollen beskriver studiet av nøytrofil-biofilm-interaksjoner. Staphylococcus aureus biofilmer er etablert in vitro og inkuberes med perifere blodavledede humane nøytrofiler. Den oksidative burstresponsen fra nøytrofiler kvantifiseres, og nøytrofillokaliseringen i biofilmen bestemmes ved mikroskopi.

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Immunologi og infeksjon utgave 184 biofilm nøytrofiler ROS-produksjon mikroskopi vertspatogene interaksjoner
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