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Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion
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Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion
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Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion

DOI:
10.3791/63788-v

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07:21 min

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July 07, 2023

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,
1Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine,Oslo University Hospital, 2Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences,University of Oslo

Chapters

  • 00:00Introduction
  • 00:38Cultivation of Human Fibroblast Cells and Preparation of the Feeder Cell Layer
  • 03:42Harvesting hESC or hiPSC Colonies Using EDTA‐Mediated Dis-Adhesion
  • 05:15Results: Comparison of EDTA‐Mediated Dis‐Adhesion and Mechanical Harvesting of hESCs Cultured on Feeder Cells
  • 06:40Conclusion

Summary

Automatic Translation

Automatic Translation

To avoid the limitations associated with the enzymatic or mechanical passaging of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) cultured on feeder cells, we have established a fast, effective, cost-efficient, high-yield method for harvesting hESC or hiPSC colonies maintained on a feeder cell layer of human foreskin fibroblasts using EDTA-mediated dis-adhesion.

Tags

Keywords: Human Pluripotent Stem CellsFeeder CellsEnzyme-free PassagingEDTA-mediated Dis-adhesionRapidCost-efficientMitotic ArrestGamma Irradiation

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