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Analyse de la morphologie organoïde rectale (ROMA)
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Analyse de la morphologie organoïde rectale (ROMA): un test diagnostique dans la fibrose kystique

Article DOI: 10.3791/63818-v • 07:56 min • June 10th, 2022
June 10th, 2022 •
1,2, 1, 3,4, 5, 6, 7,8, 7,9, 8, 3,4, 1,2, 1,2, 10,11, 1,2, 1,2
1Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, 3VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience, KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, 9Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, 11Department of Respiratory Diseases, University Hospitals Leuven

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Ce protocole décrit l’analyse de la morphologie organoïde rectale (ROMA), un nouveau test diagnostique de la fibrose kystique (FK). Les caractéristiques morphologiques, à savoir la rondeur (indice de circularité, IC) et la présence d’une lumière (rapport d’intensité, IR), sont une mesure de la fonction CFTR. L’analyse de 189 sujets a montré une discrimination parfaite entre les FK et les non-FC.

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Médecine numéro 184
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