Forskoline induite par Gonflement dans Intestinal organites: Un<em> In vitro</em> Essai pour évaluer la réponse des médicaments dans les patients atteints de fibrose kystique
This protocol describes an assay for measuring CFTR function and CFTR modulator responses in cultured tissue from subjects with cystic fibrosis (CF). Biopsy-derived intestinal organoids swell in a cAMP-driven fashion, a response that is defective (or strongly reduced) in CF organoids and can be restored by exposure to CFTR modulators.