JoVE Journal
Developmental Biology
Developmental Biology
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Summary
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该协议提出了一种从解剖的小鼠胚胎面部过程或培养的小鼠胚胎腭间充质细胞中分离全细胞蛋白裂解物并随后进行蛋白质免疫印迹以评估磷酸化蛋白水平的方法。