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Developmental Biology
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原代小鼠胚胎腭间充质细胞的分离和延时成像,以分析集体运动属性
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JoVE Journal
Developmental Biology
Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
Please note that all translations are automatically generated.
Click here for the English version.
原代小鼠胚胎腭间充质细胞的分离和延时成像,以分析集体运动属性
DOI:
10.3791/62151-v
•
07:13 min
•
February 13, 2021
•
Jeremy P. Goering*
1
,
Dona Greta Isai*
1
,
Andras Czirok
2
,
Irfan Saadi
1
Department of Anatomy and Cell Biology
,
University of Kansas Medical Center
,
2
Department of Biological Physics
,
Eotvos University
Chapters
00:05
Introduction
00:38
Embryonic Palatal Shelf Dissection
02:54
Mouse Embryonic Palatal Mesenchymal (MEPM) Cell Culture
04:07
2D Collective Migration Assay Setup
04:52
Wound Repair Assay Setup
05:28
Results: Representative MEPM Imaging and Collective Movement Analysis
06:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一种分离和培养原代小鼠胚胎腭间充质细胞的方案,用于二维(2D)生长和伤口修复测定的延时成像。我们还提供了分析延时成像数据的方法,以确定细胞流形成和方向运动。
Tags
Primary Mouse Embryonic Palatal Mesenchyme Cells
Time-lapse Imaging
Collective Movement Attributes
Palatal Shelf Elevation
Craniofacial Field
Mesenchymal Remodeling
Embryonic Day 13.5
Palatal Shell
MEPM Culture Medium
Fine Forceps
Stainless Steel Scissors
Cell Isolation
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