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Isolering af helcelleproteinlysater fra musens ansigtsprocesser og dyrkede palatale mesenchymceller til fosfoproteinanalyse
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Isolering af helcelleproteinlysater fra musens ansigtsprocesser og dyrkede palatale mesenchymceller til fosfoproteinanalyse

DOI:
10.3791/63834-v

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07:26 min

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April 01, 2022

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, ,
1Department of Craniofacial Biology, School of Dental Medicine,University of Colorado Anschutz Medical Campus

Chapters

  • 00:04Introduction
  • 00:48Harvesting E11.5 Mouse Embryos
  • 02:31Dissecting Maxillary Processes from E11.5 Mouse Embryos
  • 04:12Isolating Whole Cell Protein Lysates from Mouse Maxillary Processes
  • 05:06Isolating Whole Cell Protein Lysates from Primary and/or Immortalized Mouse Embryonic Palatal Mesenchyme Cells
  • 06:22Results: Assessing Phosphorylated Protein Levels in Cell Lysates from Cultured Mouse Embryonic Palatal Mesenchyme Cells
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

Protokollen præsenterer en metode til isolering af helcelleproteinlysater fra dissekerede museembryo-ansigtsprocesser eller dyrkede museembryonale palatale mesenchymceller og udfører efterfølgende western blotting for at vurdere fosforylerede proteinniveauer.

Tags

Protein LysatesPhosphoprotein AnalysisFacial ProcessesPalatal Mesenchyme CellsCraniofacial DevelopmentMouse EmbryosProtein IsolationPhosphatase InhibitorsProtein PhosphorylationDissectionCell Culture

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