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Messung von Proteinumsatzraten in seneszenten und nicht teilenden kultivierten Zellen mit metabolischer Markierung und Massenspektrometrie
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Chapters
- 00:05Introduction
- 00:58Labeling of Cells Pulsed SILAC and Harvest of Lysates
- 02:29In-Solution Trypsin Digestion and Sample Clean-Up with Solid-Phase Extraction (SPE)
- 04:15Data-Dependent Acquisition Mass Spectrometry Analysis and Calculation of Protein Half-Lives
- 06:31Results: Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells Using pSILAC Mass Spectrometry
- 08:13Conclusion
