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Monstervoorbereiding voor snelle lipidenanalyse in drosophila-hersenen met behulp van matrixondersteunde laserdesorptie / ionisatie massaspectrometrie beeldvorming
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Monstervoorbereiding voor snelle lipidenanalyse in drosophila-hersenen met behulp van matrixondersteunde laserdesorptie / ionisatie massaspectrometrie beeldvorming

Article DOI: 10.3791/63930-v • 09:00 min • July 14th, 2022
July 14th, 2022 •
*1,5,6, *1,2, *3, 1,4, 7, 1,4,5, 1,4,5, 8, 9,10, 2, 1
1Advanced Science Research Center, Neuroscience Initiative, the City University of New York, Graduate Center New York, 2Advanced Science Research Center, Structural Biology Initiative, the City University of New York, Graduate Center, 3National Institute of Neurological Disorders and Stroke, National Institutes of Health, 4The City College of New York, CUNY, 5Macaulay Honors College, CUNY, 6Graduate School of Public Health and Health Policy, The City University of New York, 7Princeton University, 8Ardrey Kell High School, 9The Borough of Manhattan Community College, CUNY, 10Gallatin School of Individualized Study, New York University
* These authors contributed equally

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Het doel van dit protocol is om gedetailleerde richtlijnen te geven over de juiste monstervoorbereiding voor lipide- en metabolietanalyse in kleine weefsels, zoals de Drosophila-hersenen , met behulp van matrixondersteunde laserdesorptie / ionisatie (MALDI) massaspectrometrie beeldvorming.

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Biologie MALDI MSI lipid profiling Drosophila brain sample preparation mass spectrometry
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