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1Advanced Science Research Center, Neuroscience Initiative,the City University of New York, Graduate Center New York, 2Advanced Science Research Center, Structural Biology Initiative,the City University of New York, Graduate Center, 3National Institute of Neurological Disorders and Stroke,National Institutes of Health, 4The City College of New York,CUNY, 5Macaulay Honors College,CUNY, 6Graduate School of Public Health and Health Policy,The City University of New York, 7Princeton University, 8Ardrey Kell High School, 9The Borough of Manhattan Community College,CUNY, 10Gallatin School of Individualized Study,New York University
Chapters
- 00:04Introduction
- 00:46Fly Head Embedding
- 02:59Cryosectioning the Tissue
- 07:12Results: MALDI MS Analysis and Mass Spectra of OCT and Drosophila Brain Tissue
- 08:11Conclusion
Ziel dieses Protokolls ist es, detaillierte Anleitungen zur richtigen Probenvorbereitung für die Lipid- und Metabolitenanalyse in kleinen Geweben wie dem Drosophila-Gehirn unter Verwendung der matrixgestützten Laserdesorption / Ionisation (MALDI) Massenspektrometrie bereitzustellen.
