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Journal / Genetics / Demonstration af sekvensjusteringen for at…
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Genetics

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JoVE Journal Genetics
Demonstration af sekvensjusteringen for at forudsige følsomhedsværktøj på tværs af arter til hurtig vurdering af proteinbevarelse
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Demonstration af sekvensjusteringen for at forudsige følsomhedsværktøj på tværs af arter til hurtig vurdering af proteinbevarelse

Article DOI: 10.3791/63970-v • 16:02 min • February 10th, 2023
February 10th, 2023 •
1, 2, 2, 3, 4, 5, 5, 5, 6
1Office of Research and Development, Center for Computational Toxicology and Exposure, Scientific Computing and Data Curation Division, U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education, 3Swenson College of Science and Engineering, Department of Biology, University of Minnesota Duluth, 4University of Wisconsin-Madison Aquatic Sciences Center, 5General Dynamics Information Technology, Research Triangle Park, 6Office of Research and Development, Center for Computational Toxicology and Exposure, Great Lakes Toxicology and Ecology Division, U.S. Environmental Protection Agency

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Her præsenterer vi en protokol til at bruge den nyeste version af US Environmental Protection Agency Sequence Alignment to Predict Across Species Susceptibility (SeqAPASS) værktøj. Denne protokol demonstrerer anvendelsen af onlineværktøjet til hurtigt at analysere proteinbevarelse og give tilpasselige og let fortolkelige forudsigelser af kemisk modtagelighed på tværs af arter.

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