00:56Developing and Running a SeqAPASS Query: Level 1
02:43Developing and Running a SeqAPASS Query: Level 2
03:47Accessing and Understanding the Data: SeqAPASS Level 1 and Level 2
05:54Manipulating Data Settings and Visualizing the Data: SeqAPASS Level 1 and Level 2
07:23Developing and Running a SeqAPASS Analysis: Level 3
08:28Identify Critical Amino Acid Residues through a Literature Search
10:54Visualizing Level 3 SeqAPASS Data and Interpretation of Results
13:45Results: SeqAPASS Level 1, 2, and 3 Analyses of the Conservation of Amino Acid Residues and Level 1 and 2 Analyses of μ Opioid Receptor Conservation Across Taxonomic Groups
Hier stellen wir ein Protokoll vor, um die neueste Version des SeqAPASS-Tools (Sequence Alignment to Predict Across Species Susceptibility) der US Environmental Protection Agency zu verwenden. Dieses Protokoll demonstriert die Anwendung des Online-Tools zur schnellen Analyse der Proteinkonservierung und zur Bereitstellung anpassbarer und leicht interpretierbarer Vorhersagen der chemischen Empfindlichkeit über Spezies hinweg.