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JoVE Journal Bioengineering
Fabrication et utilisation d’échafaudages d’alginate macroporeux secs pour la transduction virale des lymphocytes T
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Fabrication et utilisation d’échafaudages d’alginate macroporeux secs pour la transduction virale des lymphocytes T

Article DOI: 10.3791/64036-v • 11:16 min • September 9th, 2022
September 9th, 2022 •
1, 1,2, 1,2, 1,2,3
1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, 2Comparative Medicine Institute, North Carolina State University, 3Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill

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Voici un protocole pour créer des échafaudages d’alginate macroporeux secs qui interviennent dans le transfert efficace de gènes viraux pour une utilisation dans le génie génétique des cellules T, y compris les cellules T pour la thérapie cellulaire CAR-T. Il a été démontré que les échafaudages transduisaient les lymphocytes T primaires activés avec une transduction de >85%.

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Bioingénierie numéro 187 échafaudage de biomatériaux alginate macroporeux transduction virale immunothérapie cellules CAR-T
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