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Fremstilling af nukleosomkernepartikler kompleksbundet med DNA-reparationsfaktorer til kryo-elektronmikroskopi strukturbestemmelse…
JoVE Journal
Biochemistry
Biochemistry
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Fremstilling af nukleosomkernepartikler kompleksbundet med DNA-reparationsfaktorer til kryo-elektronmikroskopi strukturbestemmelse
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Article
DOI:
10.3791/64061-v
•
07:59 min
•
August 17th, 2022
Fremstilling af nukleosomkernepartikler kompleksbundet med DNA-reparationsfaktorer til kryo-elektronmikroskopi strukturbestemmelse
August 17th, 2022
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* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
Denne protokol beskriver detaljeret fremstillingen af nukleosomale komplekser ved anvendelse af to metoder til prøveforberedelse til frysning af TEM-gitter.