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Immunology and Infection

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JoVE Journal Immunology and Infection
Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs
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Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs

Article DOI: 10.3791/64089-v • 08:44 min • June 23rd, 2022
June 23rd, 2022 •
1, 1, 2, 1,3, 4, 1,3
1Department of Surgery, Washington University School of Medicine, 2Department of Surgery, The University of Maryland, 3Department of Pathology & Immunology, Washington University School of Medicine, 4Department of Medicine, Washington University School of Medicine

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Summary
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Mouse cardiac transplantation models represent valuable research tools for studying transplantation immunology. The present protocol details mouse heterotopic cervical cardiac transplantation that involves the placement of cuffs on the recipient's common carotid artery and the donor's pulmonary artery trunk to allow for laminar blood flow.

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Mouse Heterotopic Cervical Cardiac Transplantation Vascular Cuffs Laminar Blood Flow Donor Pulmonary Artery Turbulent Blood Flow Thrombus Formation Survival Rates Immune Responses Ischemia Reperfusion Injury Graft Rejection Post-transplant Monitoring Re-transplantation Surgical Area Preparation Heparin Administration Supine Position Surgical Tape Disinfection Incision Median Laparosternotomy Bilateral Thoracotomy Anterior Chest Wall Folding Mediastinum Exposure Thymus Excision Inferior Vena Cava Exposure Retrograde Perfusion
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