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Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs
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Chapters
- 00:04Introduction
- 01:01Donor Procedure
- 03:39Recipient Procedure
- 07:15Results: Using Vascular Cuffs for Heterotopic Cervical Cardiac Transplantation in Mice
- 08:12Conclusion
Mouse cardiac transplantation models represent valuable research tools for studying transplantation immunology. The present protocol details mouse heterotopic cervical cardiac transplantation that involves the placement of cuffs on the recipient's common carotid artery and the donor's pulmonary artery trunk to allow for laminar blood flow.
Tags
Mouse Heterotopic Cervical Cardiac TransplantationVascular CuffsLaminar Blood FlowDonor Pulmonary ArteryTurbulent Blood FlowThrombus FormationSurvival RatesImmune ResponsesIschemia Reperfusion InjuryGraft RejectionPost-transplant MonitoringRe-transplantationSurgical Area PreparationHeparin AdministrationSupine PositionSurgical TapeDisinfectionIncisionMedian LaparosternotomyBilateral ThoracotomyAnterior Chest Wall FoldingMediastinum ExposureThymus ExcisionInferior Vena Cava ExposureRetrograde Perfusion
