Partial Heterotopic Hindlimb Transplantation Model in Rats

Marion Goutard; Mark A. Randolph; Corentin B. Taveau; Elise Lupon; Laurent Lantieri; Korkut Uygun; Curtis L. Cetrulo Jr.; Alexandre G. Lellouch

doi:10.3791/62586

Partial Heterotopic Hindlimb Transplantation Model in Rats

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Partial Heterotopic Hindlimb Transplantation Model in Rats

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06:19 min

June 9, 2021

DOI: 10.3791/62586-v

Marion Goutard^1,2,3, Mark A. Randolph^1,2,3, Corentin B. Taveau^1,2,3,4, Elise Lupon^1,2,3, Laurent Lantieri⁴, Korkut Uygun^1,2,3, Curtis L. Cetrulo Jr.*^1,2,3, Alexandre G. Lellouch*^1,2,3,4

¹Division of Plastic and Reconstructive Surgery,Massachusetts General Hospital, Harvard Medical School, ²Vascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences,Massachusetts General Hospital, Harvard Medical School, ³Shriners Hospital for Children, ⁴Service de Chirurgie Plastique, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris (APHP),Université de Paris

This paper presents a partial heterotopic osteomyocutaneous flap transplantation protocol in rats and its potential outcomes in the mid-term follow-up.

We present a reliable and reproducible surgical model of heterotopic hindlimb transplantation in rats. This technique is ideal for short-term studies on preservation or immune rejection in VCA. The step-by-step description will allow trainees to rapidly master the surgery.

To begin, make a circumferential incision on the skin above the ankle at the distal third of the leg. Skeletonize and cauterize the saphenous artery and the terminal branch of the popliteal artery using bipolar forceps. Cauterize and cut the gastrocnemius, soleus, tibialis anterior, and biceps femoris muscles until the tibial bone is exposed.

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