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Combinación de actuador de fuerza magnética 3D e imágenes de fluorescencia multifuncionales para estudiar la mecanobiología del núcleo

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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Este estudio presenta un nuevo protocolo para aplicar directamente fuerza mecánica en el núcleo celular a través de microperlas magnéticas entregadas en el citoplasma y para realizar imágenes fluorescentes simultáneas de células vivas.

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Biología Número 185 Mecanobiología del núcleo fuerza magnética CRISPR/Cas9 mecanotransducción materia activa blanda proteína asociada al sí (YAP) imágenes funcionales fluorescentes procesamiento digital de imágenes optogenética epigenética
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