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Journal / Biology / 3D磁力アクチュエータと多機能蛍光イメージングを組み合わせて核メカノバイオロジーを研究…
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3D磁力アクチュエータと多機能蛍光イメージングを組み合わせて核メカノバイオロジーを研究
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3D磁力アクチュエータと多機能蛍光イメージングを組み合わせて核メカノバイオロジーを研究

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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本研究では、細胞質内に送達される磁気マイクロビーズを介して細胞核に直接力を加え、生細胞蛍光イメージングを同時に行うための新しいプロトコルを提示します。

Tags

生物学、185号、核メカノバイオロジー、磁力、CRISPR/Cas9、メカノトランスダクション、ソフトアクティブマター、はい随伴タンパク質(YAP)、蛍光機能イメージング、デジタルイメージング処理、オプトジェネティクス、エピジェネティクス
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