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JoVE Journal Biology
Combinando o Atuador de Força Magnética 3D e a Imagem de Fluorescência Multifuncional para Estudar a Mecanologia do Núcleo
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Combinando o Atuador de Força Magnética 3D e a Imagem de Fluorescência Multifuncional para Estudar a Mecanologia do Núcleo

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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Este estudo apresenta um novo protocolo para aplicar diretamente força mecânica no núcleo celular através de microesferas magnéticas entregues no citoplasma e para realizar imagens fluorescentes simultâneas de células vivas.

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Biologia Edição 185 Nucleus mechanobiology magnetic force CRISPR/Cas9 mechanotransduction soft active matter yes-associated protein (YAP) fluorescente functional imaging digital imaging processing optogenetics epigenetics
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