Journal
/
Biology
/
A Proximal Culture Method to Study Paracrine Signaling Between Cells
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
A Proximal Culture Method to Study Paracrine Signaling Between Cells

A Proximal Culture Method to Study Paracrine Signaling Between Cells

DOI:
10.3791/58144-v

•

08:17 min

•

August 28, 2018

•

, , , ,
1Indiana University School of Medicine, 2Urology,Indiana University Health Southern Indiana Physicians, 3Indiana University Melvin and Bren Simon Cancer Center, 4Department of Medical and Molecular Genetics,Indiana University School of Medicine

Chapters

  • 00:04Title
  • 01:01Proximal Culture Setup
  • 03:46RNA Extraction
  • 05:37Results: Representative Proximal Culture Effects on mRNA Expression
  • 07:31Conclusion

Summary

Automatic Translation

Automatic Translation

Paracrine and juxtacrine cellular interactions play an important role in many biological processes, including tumor progression, immune responses, angiogenesis, and development. Here, a proximal culture method is used to study paracrine signaling where the localized concentrations of the secreted factors are maintained while preventing direct cellular contact.

Tags

Keywords: Paracrine SignalingIntercellular CommunicationProximal Culture MethodCancer CellsTumor MicroenvironmentOvarian Cancer CellsHPMCJuxtacrine SignalingCell CultureCell Interactions

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58144?article_type=v

Testing Visual Sensitivity to the Speed and Direction of Motion in Lizards
check_url/58144?article_type=v

Method for Culture of Early Chick Embryos ex vivo (New Culture)
check_url/58144?article_type=v

A high-throughput method to globally study the organelle morphology in S. cerevisiae
check_url/58144?article_type=v

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
check_url/58144?article_type=v

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt
check_url/58144?article_type=v

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
check_url/58144?article_type=v

Visualization of Vascular Ca2+ Signaling Triggered by Paracrine Derived ROS
check_url/58144?article_type=v

A High-content Imaging Workflow to Study Grb2 Signaling Complexes by Expression Cloning
check_url/58144?article_type=v

A Novel Ex vivo Culture Method for the Embryonic Mouse Heart
check_url/58144?article_type=v

The Slice Culture Method for Following Development of Tooth Germs In Explant Culture

Read Article