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Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

DOI:
10.3791/64103-v

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08:12 min

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August 23, 2022

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, ,
1The University of Queensland Diamantina Institute,The University of Queensland, 2School of Mathematical Sciences,Queensland University of Technology

Chapters

  • 00:04Introduciton
  • 00:43Preparation of 2% Agarose‐PBS Gel and Clearing Solution
  • 01:39Spheroid Preparation and Staining
  • 04:04Spheroid Mounting and Imaging
  • 05:46Results: Semi-High‐Throughput Imaging of 3D Tumor Spheroids
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Tumor spheroids are becoming increasingly utilized to assess tumor cell-microenvironment interactions and therapy response. The present protocol describes a robust but simple method for semi-high-throughput imaging of 3D tumor spheroids using rapid optical clearing.

Tags

Keywords: Tumor Spheroids3D Tumor ModelingOptical ClearingRapid ClearingSemi-high-throughput AnalysisAgarose Overlay MethodParaformaldehyde FixationAntibody StainingTriton X-100 Permeabilization

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